^{Bian Yue, Sun Yi, Li Chaoxia, Zhu Kai, Sun Guangchao, Yang Zaibin}

Shandong Agricultural University

 SD BIOTECH GROUP 

Abstract: In order to study the effect of Boluohui extract on the production performance of commercial broiler chickens. 720 1-day-old AA broiler chickens were selected for the experiment and randomly divided into 8 treatments, with 6 replicates per treatment and 15 chickens per replicate. Using a 2 × 4 factorial design, i.e., with and without invasion of Clostridium difficile, four treatments were set up on the basis of invasion and non invasion, respectively, with the addition of Boluohui extract at 0 mg/kg, 200 mg/kg, 400 mg/kg, and 800 mg/kg. Try the chicken cage and freely eat and drink. The experimental period is 42 days. The results showed that: 1) Compared with not using Clostridium difficile for infection, using Clostridium difficile for infection reduced daily weight gain to a certain extent and improved feed to weight ratio, but the effect was not significant (p>0.05), indicating that the establishment of a necrotic enteritis model has been basically successfully completed through infection experiments. 2) The addition of Boluohui extract improved the production performance of commercial broiler chickens to a certain extent. Under the conditions of this experiment, the addition level of 400 mg/kg showed the best production performance. This indicates that the establishment of a necrotic enteritis model can be achieved by attacking with Clostridium difficile; Adding Boluohui extract to the diet of commercial broiler chickens can improve production performance to a certain extent.

Keywords: Boluohui extract, broiler, production performance, daily weight gain, feed to meat ratio

1 Materials and Methods

1.1 Test Materials

Boluohui extract:Bai Qu Cai Hong alkaline ≥ 900 mg/kg, Xue Gen alkaline ≥ 1500 mg/kg. Provided by Shengdao Biotechnology (Shandong) Co., Ltd.

Clostridium perfringens，purchased from BNCC, number 185933. After activation, carry out enrichment culture (both activation and enrichment culture are carried out in the technical center of Mount Taishan Shengliyuan Group Co., Ltd.). After 12 hours, stop the culture and count. The cell concentration is about 3 × 108 CFU/mL, repeat three times, and the concentration is stable.

1.2 Experimental animals and design

720 healthy AA broiler chickens aged 1 day were selected and randomly divided into 8 treatments, with 6 replicates per treatment and 15 chickens per replicate. Using a 2 × 4 factorial design, i.e. Clostridium difficile (attack vs. no attack), four treatments were set up on the basis of attack and no attack, respectively, with the addition of Boluohui extract at 0 mg/kg, 200 mg/kg, 400 mg/kg, and 800 mg/kg. Try raising chickens in cages and have free access to food and water. At 14 days of age, the attacking group was fed with 50 mL of Clostridium difficile bacterial solution containing approximately 3.0 × 108 CFU/mL evenly sprayed onto a 250g diet every 15 days (each animal was fed with 1 × 109 CFU/mL). The next day (at 15 days of age), the same amount of bacterial solution was given again to establish a chicken necrotic enteritis model. Healthy chickens were given 50mL of liquid culture medium every 15 as a control.

1.3 Measurement indicators

During the experiment, the material consumption was counted daily on a repeated basis, and the weight was measured on an empty stomach at 21 and 42 days. Calculate the average daily feed intake, average daily weight gain, and feed to gain ratio.

1.4 Data statistics and analysis

The experimental data was analyzed using the GLM process of SAS 9.2 software, and multiple comparisons were conducted using Duncan's method, with p<0.05 as the criterion for significant differences.

2 Results and Analysis

The effects of Boluohui extract on the production performance of commercial broiler chickens are shown in Tables 1 and 2. According to Tables 1 and 2, the use of Clostridium difficile for toxin attack had no significant effect on the production performance at each stage (p>0.05), while the addition of Boluohui only significantly reduced the feed to weight ratio from 1-21d (p<0.01), and="" had="" no="" significant="" effect="" on="" other="" production="" performance="" indicators="" at="" each="" stage="" p="">0.05).

Compared to not using Clostridium difficile for poisoning, using Clostridium difficile for poisoning. On days 1-21, daily weight gain decreased by 0.2%, feed intake increased by 1.6%, feed to gain ratio increased by 1.7%, and body weight decreased by 0.2% on day 21; From 22 to 42 days, daily weight gain decreased by 1.4%, feed intake decreased by 0.5%, feed to gain ratio increased by 1.2%, and body weight decreased by 1.4% at 42 days; From day 1 to day 42, daily weight gain decreased by 1.0%, feed intake remained unchanged, and feed to gain ratio increased by 1.2%. This indicates that the establishment of a necrotic enteritis model by attacking with Clostridium difficile is basically successful.

3 Conclusion

3.1 Commercial broiler chickens at 14 days of age were subjected to a challenge test using Clostridium difficile, resulting in a decrease in daily weight gain and an increase in feed to weight ratio at each stage, indicating that the establishment of a necrotic enteritis model through challenge was basically successful.

3.2 Boluohui extract can improve the production performance of commercial broiler chickens to a certain extent, with 400 mg/kg showing good production performance.

Comparison of the effects of Boluohui extract on the production performance of broiler chickens in Table 1

Table 2 Relative comparison of the effects of Boluohui extract on the production performance of commercial broiler chickens,%