A Brief Report on the Comparative Study of the Application Efficacy of Yiningyi Commercial Broiler - Scientific Payoffs

Zhu Kaiqu Haojie, Sun Guangchao, Li Zhaoyong, Zhang Mingquan, Yang Zaibin

Shandong Agricultural University, SD BIOTECH GROUP

Abstract: In order to study the efficacy of Yiningyi in improving the production performance, nutrient and energy utilization efficiency, and meat production performance of commercial broiler chickens. 2240 1-day-old healthy AA broiler chickens were selected for the experiment and randomly divided into 2 treatments, with 5 replicates per treatment and 224 chickens per replicate. Treatment 1: Control group, fed with basic diet; Treatment 2: In the experimental group, a 50g/ton level of Yiningyi was added to the basic diet of the control group in treatment 1. Try raising chickens in cages, freely consume food and water, and feed them with pellet feed. It is divided into two stages: 0-21 days and 22-42 days. The results indicate that:

The results of production and meat performance showed that compared with the control group, adding Yiningyi to the diet significantly increased the body weight (21 days, 42 days) and daily weight gain (1-21 days, 22-42 days, 1-42 days) of broiler chickens (P<0.05), reduced the feed to weight ratio (1-21 days, 22-42 days, 1-42 days) (P<0.05), and="" had="" no="" significant="" effect="" on="" daily="" feed="" intake="" 1-21="" 22-42="" 1-42="" p="">0.05). Compared with the control group, the addition of Yiningyi to the diet had no significant effect on the meat production performance indicators of broiler chickens at 21 and 42 days (P>0.05).

The results of liver nutrient content showed that compared with the control group, the addition of Yiningyi to the diet significantly reduced the crude protein content of fresh basal liver after 21 days (P<0.05), 21="" 42="" had="" no="" significant="" effect="" on="" the="" dry="" matter="" based="" liver="" organic="" crude="" and="" fat="" content="" p="">0.05), and had no significant effect on the dry matter, organic matter, carbohydrates, and crude fat content of fresh basal liver (21 days, 42 days) (P>0.05).

Comparative analysis of nutrient and energy utilization efficiency: Compared with the control group, adding Yiningyi to the diet significantly improved the apparent and true utilization efficiency of dry matter, organic matter, crude protein, crude fat, and total energy (P<0.05), but="" did="" not="" significantly="" affect="" the="" apparent="" and="" true="" utilization="" efficiency="" of="" carbohydrates="" p="">0.05) or the apparent and true metabolic energy values (P>0.05). Compared with the control group, the addition of Yiningyi to the diet significantly increased the apparent utilization rates of tryptophan, arginine, serine, glycine, alanine, tyrosine, and total non essential amino acids (P<0.05), significantly increased the true utilization rates of lysine, methionine, tryptophan, histidine, isoleucine, phenylalanine, threonine, valine, aspartic acid, glutamic acid, alanine, cysteine, tyrosine, and total essential amino acids (P<0.05), and="" had="" no="" significant="" effect="" on="" the="" apparent="" true="" utilization="" rates="" of="" leucine="" proline="" p="">0.05).

Keywords: Yiningyi, commercial broiler, production performance, meat production performance, nutrients, energy

1 Materials and Methods

1.1 Test Materials

Yiningyi: Composed of brewing yeast extract and brewing yeast culture (crude protein ≥ 30.0%, crude fat ≥ 4.0%, crude fiber ≤ 1.0%, crude ash ≤ 20.0%, moisture ≤ 12.0%, mannan ≥ 6.0%), provided by Cherry Dewey Co., Ltd. in the United States.

1.2 Experimental animals and design

2240 healthy AA broiler chickens aged 1 day were selected and randomly divided into 2 treatments, with 5 replicates per treatment and 224 chickens per replicate. Treatment 1: Control group, fed with basic diet; Treatment 2: In the experimental group, 500 mg/kg of Yiningyi was added to the basic diet of the control group in treatment 1. Try raising chickens in cages, freely consume food and water, and feed them with pellet feed. It is divided into two stages: 0-21 days and 22-42 days. The composition and nutritional level of the basic diet are shown in Table 1.

1.3 Measurement indicators

Production performance: During the experiment, the feed intake was recorded daily on a repeated basis, with 21 days (measured by random weight, 20% chickens) and 42 days (all weighed). Calculate the average daily feed intake, average daily weight gain, and feed to gain ratio. Record mortality and diarrhea rates.

Meat production performance: At 21 and 42 days, 10 animals (2 per replicate) were selected for slaughter in each treatment, and semi clean bore rate, full clean bore rate, abdominal fat rate, leg muscle rate, and chest muscle rate were measured.

Physiological indicators: At 21 and 42 days, abdominal fat percentage and liver fat content were measured using a test chicken that measured meat production performance.

Nutrient and energy utilization efficiency: 28-35 days, determine the nutrient utilization efficiency of the diet using the full manure collection method. At the same time, an additional 20 animals were randomly divided into 5 replicates, with 4 animals in each replicate. Fasting was carried out for 24 hours, and the endogenous nutrient excretion in the digestive tract was measured to calculate the true utilization rate of nutrients.

Liver nutrient composition: 21 days and 42 days, measured using a test chicken for meat production performance to determine liver nutrient composition

Economic benefits: Comparison of feed benefits (European index).

1.4 Data statistics and analysis

The data was analyzed using SAS 9.2 software, and Duncan's multiple range test was used to compare the mean values, with a significance level of P<0.05.

2 Results and Analysis

2.1 Comparative analysis of production performance

The effect of Yiningyi on the production performance of broiler chickens is shown in Table 2. Compared with the control group, adding Yiningyi to the diet significantly increased the body weight (21 days, 42 days) and daily weight gain (1-21 days, 22-42 days, 1-42 days) of broiler chickens (P<0.05), significantly reduced the feed to weight ratio (1-21 days, 22-42 days, 1-42 days) (P<0.05), and="" had="" no="" significant="" effect="" on="" the="" daily="" feed="" intake="" 1-21="" 22-42="" 1-42="" of="" broiler="" chickens="" p="">0.05).

2.2 Comparative analysis of meat production performance

The effects of Yiningyi on the meat production performance of broiler chickens are shown in Tables 3 and 4. Compared with the control group, the addition of Yiningyi to the diet had no significant effect on the meat production performance of broiler chickens at 21 and 42 days (P>0.05).

2.3 Comparative analysis of liver components

The effects of Yiningyi on the nutritional components of broiler liver are shown in Tables 5 and 6. Compared with the control group, the addition of Yiningyi to the diet significantly increased the crude protein content of fresh basal liver for 21 days (P<0.05), 21="" 42="" but="" had="" no="" significant="" effect="" on="" the="" dry="" matter="" organic="" crude="" and="" fat="" content="" p="">0.05), and had no significant effect on the dry matter, organic matter, carbohydrates, and crude fat content of fresh basal liver for broiler chickens (21 days, 42 days) (P>0.05).

2.4 Comparative analysis of nutrient and energy utilization efficiency

Conventional Nutrients and Energy: The utilization efficiency of conventional nutrients and energy in broiler chickens by Yiningyi is shown in Tables 7 and 8. Compared with the control group, the addition of Yiningyi to the diet significantly increased the apparent and true utilization efficiency of dry matter, organic matter, crude protein, crude fat, and total energy (P<0.05), but="" had="" no="" significant="" effect="" on="" the="" apparent="" and="" true="" utilization="" metabolic="" energy="" value="" of="" carbohydrates="" p="">0.05).

Essential amino acids: Yiningyi significantly increased the apparent utilization rates of tryptophan and arginine (P<0.05), as well as the true utilization rates of lysine, methionine, tryptophan, histidine, isoleucine, phenylalanine, threonine, valine, and total essential amino acids (P<0.05), compared="" to="" the="" control="" group.="" addition="" of="" yiningyi="" diet="" had="" no="" significant="" effect="" on="" apparent="" and="" true="" utilization="" rates="" leucine="" p="">0.05).

Non essential amino acids: Yiningyi significantly increased the apparent utilization rates of serine, glycine, alanine, tyrosine, and total non essential amino acids in the diet compared to the control group (P<0.05). It also significantly increased the true utilization rates of aspartic acid, glutamic acid, alanine, cysteine, and tyrosine (P<0.05), but="" had="" no="" significant="" effect="" on="" the="" apparent="" and="" true="" utilization="" rates="" of="" proline="" p="">0.05).

3 Conclusion

Adding Yiningyi to the diet of commercial broiler chickens can achieve higher daily weight gain (1-21 days, 22-42 days, 1-42 days), lower feed to gain ratios (1-21 days, 22-42 days, 1-42 days), and higher slaughter weight (21 days, 42 days).

Adding Yiningyi to the diet of commercial broiler chickens cannot improve meat production performance.

Adding Yiningyi to the diet of commercial broiler chickens can result in higher liver crude protein content.

Adding Yiningyi to the diet of commercial broiler chickens can improve the true and apparent utilization rates of conventional nutrients, energy, and amino acids.

Table 1 Basic Diet Composition and Nutritional Level

Raw materials,%	1-21d	22-42d	Nutritional indicators,%	1-21d	22-42d
Corn	48.28	59.25	ME，kcal/kg	3116	3221
Flour	5.00	-	CP	21.15	19.73
Middling	6.75	1.00	Ca	0.89	0.81
Soybean meal	25.30	20.78	TP	0.58	0.52
Peanut meal	2.00	3.00	AP	0.44	0.4
Cottonseed meal	2.00	3.00	Lys	1.38	1.31
Corn gluten meal	1.00	1.00	Met	0.6	0.53
Feather Meal	1.00	1.50	M+C	0.95	0.86
CaHPO4	1.19	1.00	Thr	0.93	0.87
Stone powder	1.36	1.26	Trp	0.22	0.19
Methionine (liquid)	0.36	0.30
Lysine 70	0.79	0.89
Threonine	0.21	0.21
Soybean oil	3.55	5.50
NaCl	0.21	0.31
Premix	1.00	1.00
Amount to	100	100

Table 2: The Effect of Yiningyi on the Production Performance of Commercial Broiler Chickens

Item	1-21d				22-42d				1-42d
	ADG	ADFI	21d Weight	F/G	ADG	ADFI	42d Weight	F/G	ADG	ADFI	F/G
	g/d	g/d	g/Piece	F/G	g/d	g/d	g/Piece	F/G	g/d	g/d	F/G
Comparative analysis of production performance
Control group	43.74	56.98	960.6	1.3	89.92	145.15	2849	1.61	66.83	101.06	1.51
Yining Yi	45.46	56.97	996.7	1.25	94.09	145.38	2972.5	1.55	69.77	101.17	1.45
SEM	0.347	0.412	7.281	0.013	1.019	0.503	27.237	0.016	0.648	0.400	0.012
P-value	0.003	0.997	0.003	0.040	0.030	0.836	0.011	0.017	0.011	0.900	0.004
Relative change in production performance,%
Control group	100	100	100	100	100	100	100	100	100	100	100
Yining Yi	103.9	100.0	103.8	96.2	104.6	100.2	104.3	95.8	104.4	100.1	95.9

Table 3 Effect of Yiningyi on 21 day meat production performance of commercial broiler chickens

Item	Slaughter rate,%	Half bore rate,%	Full bore rate,%	Pectoral muscle rate,%	Leg muscle rate,%	Abdominal fat percentage,%
Comparative analysis of meat production performance,%
Control group	90.4	85.29	73.33	22.72	17.37	2.60
Yining Yi	90.86	85.84	73.55	22.91	17.75	2.99
SEM	0.249	0.433	0.402	0.221	0.416	0.203
P-value	0.368	0.543	0.791	0.689	0.662	0.357
Comparative analysis of meat production performance,%
Control group	100	100	100	100	100	100
Yining Yi	100.5	100.6	100.3	100.8	102.2	114.9

Table 4: Effect of Yiningyi on 42 day meat production performance of commercial broiler chickens,%

Item	Slaughter rate,%	Half bore rate,%	Full bore rate,%	Pectoral muscle rate,%	Leg muscle rate,%	Abdominal fat percentage,%	MSCI Europe
Comparative analysis of meat production performance
Control group	91.18	87.66	75.00	25.04	19.19	3.98	426.17
Yining Yi	91.18	87.77	75.68	25.11	19.24	4.40	470.82
SEM	0.165	0.308	0.411	0.575	0.223	0.303	8.860
P-value	0.991	0.859	0.420	0.960	0.935	0.505	0.003
Comparative analysis of meat production performance,%
Control group	100	100	100	100	100	100	100
Yining Yi	100.0	100.1	100.9	100.3	100.3	110.6	110.5

Table 5: The effect of Yiningyi on the nutritional composition of liver in commercial broiler chickens after 21 days,%

Item	Fresh sample foundation					Dry matter basis
Item	DM	OM	CP	CHO	EE	OM	CP	CHO	EE
Comparative analysis of liver nutritional components
Control group	25.03	23.62	19.27	1.27	3.08	94.37	77.04	5.07	12.26
Yining Yi	24.62	23.25	18.87	1.32	3.06	94.43	76.69	5.36	12.38
SEM	0.212	0.233	0.096	0.040	0.183	0.225	0.545	0.156	0.644
P-value	0.368	0.463	0.026	0.568	0.964	0.906	0.771	0.380	0.939
Comparative analysis of liver nutritional components,%
Control group	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Yining Yi	98.36	98.44	97.92	103.94	99.38	100.06	99.55	105.75	100.96

Table 6: Effects of Yiningyi on Liver Nutrients of Commercial Broiler Chickens at 42 Days,%

Item	Fresh sample foundation					Dry matter basis
Item	DM	OM	CP	CHO	EE	OM	CP	CHO	EE
Comparative analysis of liver nutritional components
Control group	27.50	26.04	19.42	1.36	5.26	94.66	70.68	4.96	19.03
Yining Yi	27.91	26.40	19.97	1.32	5.10	94.57	71.59	4.74	18.25
SEM	0.218	0.231	0.195	0.031	0.233	0.182	0.693	0.127	0.744
P-value	0.381	0.469	0.163	0.545	0.756	0.820	0.544	0.418	0.629
Comparative analysis of liver nutritional components,%
Control group	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Yining Yi	101.48	101.38	102.88	97.06	96.97	99.90	101.29	95.56	95.89

Table 7: Effects of Yiningyi on Conventional Nutrients and Apparent Energy Utilization Efficiency of Commercial Broiler Chickens

Item	DM，%	OM，%	CP，%	EE，%	CHO，%	GE，%	ME，MJ/kg
Comparative analysis of apparent utilization rate
Control group	80.75	81.98	62.21	85.28	86.97	81.32	13.76
Yining Yi	81.69	82.59	65.00	86.06	86.75	82.06	13.77
SEM	0.201	0.164	0.475	0.143	0.121	0.168	0.019
P-value	0.008	0.048	<0.001	<0.001	0.388	0.017	0.841
Relative comparison of apparent utilization rate,%
Control group	100	100	100	100	100	100	100
Yining Yi	101.17	100.75	104.50	100.91	99.74	100.90	100.06

Table 8: Effects of Yiningyi on the True Utilization Efficiency of Conventional Nutrients and Energy in Commercial Broiler Chickens

Item	DM，%	OM，%	CP，%	EE，%	CHO，%	GE，%	ME，MJ/kg
Comparative analysis of real utilization rate
Control group	84.32	85.64	66.22	88.45	90.21	84.74	14.34
Yining Yi	85.27	86.26	69.18	89.24	90.05	85.50	14.35
SEM	0.199	0.158	0.504	0.147	0.139	0.149	0.021
P-value	0.006	0.041	<0.001	<0.001	0.581	0.002	0.859
Compared to the actual utilization rate,%
Control group	100	100	100	100	100	100	100
Yining Yi	101.13	100.72	104.47	100.90	99.82	100.90	100.05

Table 9 Effect of Yiningyi on the apparent utilization rate of essential amino acids (EAA) in commercial broiler chickens,%

Item	Lys	Met	Trp	Arg	His	Leu	Ile	Phe	Thr	Val	TEAA
Comparative analysis of apparent utilization rate
Control group	86.69	81.28	85.44	85.78	88.34	89.30	82.38	86.40	83.64	82.63	85.16
Yining Yi	87.17	81.33	86.72	86.58	88.39	89.33	82.39	86.60	84.47	83.76	85.55
SEM	0.271	0.260	0.328	0.194	0.248	0.365	0.405	0.295	0.395	0.467	0.280
P-value	0.418	0.939	0.042	0.028	0.926	0.962	0.989	0.751	0.322	0.249	0.448
Relative comparison of apparent utilization rate,%
Control group	100	100	100	100	100	100	100	100	100	100	100
Yining Yi	100.54	100.05	101.50	100.94	100.06	100.04	100.01	100.23	100.99	101.37	100.45

Table 10: The effect of Yiningyi on the true utilization rate of essential amino acids (EAA) in commercial broiler chickens,%

Item	Lys	Met	Trp	Arg	His	Leu	Ile	Phe	Thr	Val	TEAA
Comparative analysis of real utilization rate
Control group	90.50	84.84	88.93	89.40	91.64	92.60	85.18	89.25	86.22	85.21	88.32
Yining Yi	90.99	84.88	90.26	90.22	91.71	92.62	85.18	89.52	87.07	86.36	88.72
SEM	0.271	0.288	0.334	0.197	0.252	0.369	0.411	0.295	0.400	0.475	0.283
P-value	<0.001	<0.001	<0.001	0.144	0.004	0.113	<0.001	0.001	0.001	0.044	0.040
Compared to the actual utilization rate,%
Control group	100	100	100	100	100	100	100	100	100	100	100
Yining Yi	100.54	100.05	101.49	100.92	100.34	100.03	100.00	100.30	100.98	101.35	100.45

Table 11 Effect of Yiningyi on the apparent utilization rate of non essential amino acids (EAA) in commercial broiler chickens,%

Item	Asp	Ser	Glu	Pro	Gly	Ala	Cys	Tyr	TNAA
Comparative analysis of apparent utilization rate
Control group	85.24	84.64	86.53	85.05	84.03	81.56	82.36	88.99	84.80
Yining Yi	85.98	84.90	86.53	84.70	84.71	82.95	82.38	89.08	85.15
SEM	0.398	0.364	0.267	0.292	0.771	0.577	0.610	0.241	0.260
P-value	0.254	<0.001	0.503	0.840	0.036	0.003	0.142	<0.001	<0.001
Relative comparison of apparent utilization rate,%
Control group	100	100	100	100	100	100	100	100	100
Yining Yi	100.88	100.30	99.99	99.59	100.81	101.70	100.02	100.11	100.42

Table 12: The effect of Yiningyi on the true utilization rate of non essential amino acids (EAA) in commercial broiler chickens,%

Item	Asp	Ser	Glu	Pro	Gly	Ala	Cys	Tyr	TNAA
Comparative analysis of real utilization rate
Control group	88.98	88.35	90.06	88.65	87.19	84.58	85.16	91.92	88.11
Yining Yi	89.76	88.60	90.05	88.27	87.88	86.00	85.17	92.08	88.48
SEM	0.407	0.372	0.275	0.307	0.808	0.583	0.625	0.239	0.265
P-value	0.001	0.237	<0.001	0.207	0.251	<0.001	0.002	0.024	0.518
Compared to the actual utilization rate,%
Control group	100	100	100	100	100	100	100	100	100
Yining Yi	100.87	100.29	99.99	99.57	100.79	101.68	100.02	100.17	100.41