Abstract: In order to investigate the efficacy of Yanning C1 in improving the immune and antioxidant properties of commercial broiler chickens. Select 200 healthy 1-day-old AA broiler chickens with similar body weight and randomly divide them into 2 treatments, with 5 replicates per treatment and 20 chickens per replicate (see Table 1). Treatment 1: Control group, broiler chickens were fed a regular diet (NRC recommended nutritional level) without adding any antibiotics to the diet; Treatment 2 supplemented with Yanning C1:250 mg/kg on the same diet as Treatment 1. The experiment is divided into two stages (0-21d; 22-42d). Experimental chicken cage feeding, feeding pellet feed, with consistent dietary composition and nutritional levels between treatments. At 21 and 42 days, 10 chickens were randomly selected from each treatment (2 chickens/replicate) for slaughter experiments. Blood, liver, and muscle samples were collected to measure inflammatory factors and antioxidant indicators. The measurement results indicate that:

Blood inflammatory factors: Adding Yanning C1 had no significant effect on all indicators of inflammatory factors at 21 and 42 days (P>0.05). Comparing and analyzing numerically, with the control group's various indicators at 100%, after adding Yanning C1, the serum levels of chicken tumor necrosis factor - α (IFN - α), interleukin-1 β (IL-1 β), interleukin-6 (IL-6), and interleukin-10 (IL-10) were 84.89%, 96.12%, 112.6%, and 112.99%, respectively, after 21 days of treatment; The serum levels of chicken tumor necrosis factor - α (IFN - α), interleukin-1 β (IL-1 β), interleukin-6 (IL-6), and interleukin-10 (IL-10) at 42 days were 106.33%, 99.50%, 107.21%, and 110.11%, respectively.

Antioxidant properties: Adding Yanning C1 significantly reduced the MDA content in serum, liver, and chest muscles after 21 days, significantly increased the SOD and GSH activities in serum after 21 days (P<0.05), 21="" 42="" and="" showed="" a="" trend="" of="" increasing="" the="" sod="" activity="" in="" liver="" p="" gsh="" chest="" muscles="" after="" days="" decreasing="" mda="" content="" leg="" there="" was="" no="" significant="" effect="" on="" various="" indicators="" serum="">0.05). From a numerical comparison analysis, with the control group's various indicators as 100%, the addition of Yanning C1 reduced the serum MDA content by 27.87% after 21 days, and increased SOD activity and GSH activity by 19.66% and 17.37%, respectively; After 42 days, the serum MDA content increased by 3.45%, while the SOD activity and GSH activity decreased by 3.71% and 1.18%, respectively. After 21 days, the MDA content in the liver decreased by 24.72%, while the SOD activity and GSH activity increased by 6.98% and 8.27%, respectively; After 42 days, the MDA content and SOD activity in the liver increased by 3.98% and 5.72%, respectively, while the GSH activity decreased by 14.79%. The MDA content, SOD activity, and GSH activity in the chest muscles decreased by 20.28%, 1.39%, and 11.71%, respectively, while the MDA content, SOD activity, and GSH activity in the leg muscles decreased by 13.58%, 4.63%, and 5.49%, respectively.

Keywords: commodity broiler, Yanning C1, serum inflammatory factors, antioxidant

1. Materials and Methods

1.1 Test Materials

Yanning C1: A digestive stress additive formula mainly composed of complex enzymes. Provided by SD BIOTECH GROUP

1.2 Experimental animals and design

Select 200 healthy 1-day-old AA broiler chickens with similar body weight and randomly divide them into 2 treatments, with 5 replicates per treatment and 20 chickens per replicate (see Table 1). Treatment 1: Control group, broiler chickens were fed a regular diet (NRC recommended nutritional level) without adding any antibiotics to the diet; Treatment 2 supplemented with Yanning C1:250 mg/kg on the same diet as Treatment 1. The experiment is divided into two stages (0-21d; 22-42d).

1.3 Experimental diet

Conventional diet for commercial broiler chickens in Shandong Province (see Table 2).

1.4 Test Method

Feeding experiment: The experiment is divided into two stages: broiler chickens (0-21d) and broiler chickens (22-42d). Experimental chicken cage feeding with powdered feed.

Slaughter experiment: 10 chickens were randomly selected for each treatment (2 chickens/replicate) at 21 and 42 days. Conduct slaughter experiments.

1.5 Measurement indicators

Cellular inflammatory factors: At 21 and 42 days, 5 chickens (1 per replicate) were randomly selected from each treatment for blood collection, and the expression of IL-1 β, IL-6, IL-10, and TNF - α was measured.

Blood antioxidant: At 21 and 42 days, 5 chickens were selected for each treatment, blood was collected, serum was separated, and stored at -20 ℃ to determine antioxidant indicators (MDA T-SOD、GSH-PX）。

Organ fluid antioxidant: At the end of the experimental period (slaughter), 5 chickens were selected for each treatment, and liver and chicken samples were taken to measure antioxidant indicators (MDA T-SOD、GSH-PX）。

1.6 Data statistics and analysis

The data was analyzed using SAS 9.2 software, and Duncan's multiple range test was used to compare the mean values, with a significance level of p<0.05.

2. Results and Analysis

2.1 Comparative analysis of serum inflammatory factors

The effect of supplementing 250 mg/kg of Yanning C1 in the diet of commercial broiler chickens on serum inflammatory factors is shown in Table 3. Analysis of variance showed that compared with the control group, the addition of Yanning C1 had no significant effect on various indicators of inflammatory factors at 21 and 42 days (P>0.05). Comparing and analyzing numerically, with the control group's various indicators at 100%, after adding Yanning C1, the serum levels of chicken tumor necrosis factor - α (IFN - α), interleukin-1 β (IL-1 β), interleukin-6 (IL-6), and interleukin-10 (IL-10) were 84.89%, 96.12%, 112.6%, and 112.99%, respectively, after 21 days of treatment; The serum levels of chicken tumor necrosis factor - α (IFN - α), interleukin-1 β (IL-1 β), interleukin-6 (IL-6), and interleukin-10 (IL-10) at 42 days were 106.33%, 99.50%, 107.21%, and 110.11%, respectively.

2.2 Comparative analysis of antioxidant indicators

The effects of supplementing 250 mg/kg of Yanning C1 in the diet of commercial broiler chickens on antioxidant performance are shown in Tables 4, 5, and 6. Analysis of variance showed that the addition of Yanning C1 significantly reduced the MDA content in serum, liver, and chest muscles after 21 days, significantly increased the SOD and GSH activities in serum after 21 days (P<0.05), 21="" 42="" and="" showed="" a="" trend="" of="" increasing="" the="" sod="" activity="" in="" liver="" p="" gsh="" chest="" muscles="" after="" days="" decreasing="" mda="" content="" leg="" there="" was="" no="" significant="" effect="" on="" various="" indicators="" serum="">0.05). From a numerical comparison analysis, with the control group's various indicators as 100%, the addition of Yanning C1 reduced the serum MDA content by 27.87% after 21 days, and increased SOD activity and GSH activity by 19.66% and 17.37%, respectively; After 42 days, the serum MDA content increased by 3.45%, while the SOD activity and GSH activity decreased by 3.71% and 1.18%, respectively. After 21 days, the MDA content in the liver decreased by 24.72%, while the SOD activity and GSH activity increased by 6.98% and 8.27%, respectively; After 42 days, the MDA content and SOD activity in the liver increased by 3.98% and 5.72%, respectively, while the GSH activity decreased by 14.79%. The MDA content, SOD activity, and GSH activity in the chest muscles decreased by 20.28%, 1.39%, and 11.71%, respectively, while the MDA content, SOD activity, and GSH activity in the leg muscles decreased by 13.58%, 4.63%, and 5.49%, respectively.

3. Conclusion

Compared to the control group:

Supplementing Yanning C1 in the diet of commercial broiler chickens reduced serum tumor necrosis factor - α (IFN - α) levels at 21 days, increased IFN - α levels at 42 days, reduced interleukin-1 β (IL-1 β) levels at 21 days and 42 days, and increased interleukin-6 (IL-6) and interleukin-10 (IL-10) levels.

Supplementing Yanning C1 in the diet of commercial broiler chickens has a significant improvement effect on serum and liver antioxidant indicators after 21 days. The MDA content in serum decreased by 27.87% after 21 days, and the SOD activity and GSH activity increased by 19.66% and 17.37%, respectively; After 21 days, the MDA content in the liver decreased by 24.72%, while the SOD activity and GSH activity increased by 6.98% and 8.27%, respectively; Reduced MDA content in chest and leg muscles by 20.28% and 13.58%, respectively, but had negative effects on SOD and GSH activity in muscles.

Table 1 Experimental Design

Table 2 Experimental Diet Composition and Nutritional Level

Table 3: The effect of Yanning C1 on serum inflammatory factors in commercial broiler chickens

 Table 4: The effect of Yanning C1 on serum antioxidant activity in commercial broiler chickens

Table 5: The effect of Yanning C1 on antioxidant activity in the liver of commercial broiler chickens

Table 6: The effect of Yanning C1 on antioxidant capacity of breast and leg muscles in commercial broiler chickens at 42 days