Abstract: The aim of this experiment is to investigate the efficacy of Stress Ning in improving the apparent utilization rate of amino acids in commercial broiler chickens. 1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); Process 3-4, add stress Ning 500 mg/ton on the basis of Process 1 and 2 respectively. The experiment is divided into two stages (0-21d, 22-42d). Experimental chicken cage feeding, feeding pellet feed, with consistent dietary composition and nutritional levels between treatments. The research results indicate that: 1) Essential amino acids: Reducing the nutritional level of commercial broiler diets can improve the apparent utilization of tryptophan, reduce the apparent utilization of arginine and phenylalanine (P<0.05), and have a trend of reducing the apparent utilization of lysine (P=0.074) and methionine (P=0.067); Adding stress Ning can significantly improve the apparent utilization rate of all essential amino acids (P<0.05), increasing="" the="" apparent="" utilization="" rates="" of="" and="" total="" amount="" essential="" amino="" acids="" by="" respectively.="" non="" acids:="" reducing="" nutritional="" level="" commercial="" broiler="" diets="" showed="" a="" trend="" decreasing="" efficiency="" proline="" p="" but="" had="" no="" significant="" effect="" on="" other="" indicators="">0.05); The addition of stress Ning had no significant effect on tyrosine (P>0.05), but significantly increased the apparent utilization rates of other non essential amino acids and the total amount of amino acids (P<0.05). It increased the apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, tyrosine, and the total amount of non essential amino acids by 1.3%, 6.1%, 1.7%, 11.1%, 0.7%, 1.7%, 5.5%, 0%, and 3.0%, respectively.

Keywords: commodity broiler, stress Ning, amino acid utilization rate

1. Materials and Methods

1.1 Test Materials

Stress Ning: An anti stress formula product mainly composed of star anise ether for antioxidant and immune enhancement effects, provided by SD BIOTECH GROUP.

1.2 Experimental animals and design

1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); Process 3-4, add stress Ning 500 mg/ton on the basis of Process 1 and 2 respectively. The experimental chickens were fed pellet feed and allowed to freely feed and drink.

1.3 Experimental diet

Temporarily missing

1.4 Test Method

Feeding experiment: The experiment is divided into two stages: broiler chickens (0-21d) and broiler chickens (22-42d). Experimental chicken

Caged, each replicate consists of 2 cages, with 20 animals per cage. Feed pellet feed.

1.5 Measurement indicators

Nutrient utilization efficiency:

Using the AIA indicator method, between 28-35 days, fecal samples were taken on a treatment basis to determine nutrient composition and calculate apparent utilization rate (amino acid utilization rate). (Note: During this period, select a day and before feeding in the morning. Within each replicate, collect fresh and uncontaminated feces of at least 100g per replicate, with 8 samples per treatment. At the same time, take 200g of the daily feed sample per treatment.)

1.6 Data statistics and analysis

The data was analyzed using SAS 9.2 software, and Duncan's multiple range test was used to compare the mean values, with a significance level of p<0.05.

2. Results and Analysis

2.1 Comparative analysis of apparent utilization rate of essential amino acids

Two levels of nutritional supplements (basal diet vs. reduced diet) were added to the diet of commercial broiler chickens, corresponding to two levels of stress Ning supplementation (500mg/ton without VS supplementation). The effects on the apparent utilization efficiency of essential acids are shown in Tables 2 and 3. Analysis of variance showed that reducing the nutritional level of commercial broiler diets significantly increased the apparent utilization efficiency of tryptophan, decreased the apparent utilization efficiency of arginine and phenylalanine (P<0.05), and showed a trend of decreasing the apparent utilization efficiency of lysine (P=0.074) and methionine (P=0.067); Adding stress Ning can significantly improve the apparent utilization rate of all essential amino acids (P<0.01); The nutritional level and stress tolerance showed an interactive effect on the apparent utilization rates of methionine, tryptophan, histidine, isoleucine, threonine, and valine (P<0.05), while="" there="" was="" a="" trend="" of="" interactive="" effects="" on="" arginine="" p="" isoleucine="" and="" total="" essential="" amino="" acids="" no="" effect="" lysine="" phenylalanine="">0.05). From a numerical comparison analysis, the apparent utilization rate of amino acids in the basic feed of commercial broiler chickens is 100%. After reducing the nutritional level of the diet, the apparent utilization rates of lysine, methionine, tryptophan, arginine, histidine, leucine, isoleucine, phenylalanine, threonine, valine, and essential amino acids are 99.1%, 99.1%, 101.3%, 98.2%, 99.4%, 99.5%, 99.6%, 98.7%, 99.7%, 99.5%, and 99.4%, respectively; Without adding stress Ning, the apparent utilization rates of lysine, methionine, tryptophan, arginine, histidine, leucine, isoleucine, phenylalanine, threonine, valine, and essential amino acids were 103.5%, 102.7%, 107.4%, 105.5%, 103.6%, 102.3%, 101.1%, 104.4%, 102.2%, 101.7%, and 103.4%, respectively.

From this, it can be seen that reducing the nutritional level reduces the apparent utilization rate of essential amino acids, while supplementing with stress Ning in the diet improves the apparent utilization rate of essential amino acids.

2.2 Comparative analysis of apparent utilization rates of non essential amino acids

Two levels of nutritional supplements (basal diet vs. reduced diet) were added to the diet of commercial broiler chickens, corresponding to two levels of stress Ning supplementation (500mg/ton without VS supplementation). The effects on the apparent utilization of non essential amino acids are shown in Tables 4 and 5. Analysis of variance showed that reducing the nutritional level of commercial broiler diets had a trend of decreasing the apparent utilization efficiency of proline (P=0.059), but had no significant effect on other indicators (P>0.05); The addition of stress Ning had no significant effect on tyrosine (P>0.05), but significantly increased the apparent utilization of other non essential amino acids and the total amount of amino acids (P<0.05); The nutritional level and stress Ning had an interactive effect on serine, proline, alanine, cysteine, tyrosine, and total amino acid content (P<0.05). From a numerical comparison analysis, the apparent utilization rate of non essential amino acids in the basic diet of commercial broiler chickens is 100%. After reducing the nutritional level of the diet, the apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, tyrosine, and the total amount of non essential amino acids are 99.7%, 99.6%, 99.7%, 96.1%, 100.3%, 99.5%, 100.7%, 100.0%, and 99.6%, respectively; The apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, tyrosine, and non essential amino acids were 101.3%, 106.1%, 101.7%, 111.1%, 100.7%, 101.7%, 105.5%, 100.0%, and 103.0%, respectively, with no addition of stress Ning as 100%.

3. Conclusion

The analysis results indicate that reducing the nutritional level of commercial broiler diets can lower the apparent utilization rate of amino acids, while adding stress Ning can increase the apparent utilization rate of total amino acids, which is beneficial for protein deposition in the body. Based on the comparative analysis of the four treatments, adding stress Ning to the commodity based diet can achieve the best amino acid utilization rate.

Table 1 Experimental Design

Table 2 Effect of Stress Ning on the epigenetic utilization of essential amino acids in commercial broiler chickens

Table 3 Comparison of the relative values of the effects of stress Ning on the apparent utilization rate of essential amino acids in commercial broiler chickens,%

Table 4 Effect of Stress Ning on the epigenetic utilization of non essential amino acids in commercial broiler chickens

Table 5 Comparison of the relative values of the effects of stress Ning on the apparent utilization rate of non essential amino acids in commercial broiler chickens,%