The effect of Yanning C1 on the apparent utilization rate of amino acids in commercial broiler chickens - Scientific Payoffs

Abstract:This experiment aims to investigate the efficacy of Yanning C1 in improving the apparent utilization rate of amino acids in commercial broiler chickens. 1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); For treatments 3-4, add 500 mg/ton of Yanning C1 on top of treatments 1 and 2, respectively. The experiment is divided into two stages (0-21d, 22-42d). Experimental chicken cage feeding, feeding pellet feed, with consistent dietary composition and nutritional levels between treatments. The research results indicate that: 1) Essential amino acids: Reducing the nutritional level of commercial broiler diets has a trend of increasing the apparent utilization rate of methionine (P=0.059), but has no significant effect on other indicators (P>0.05); Adding Yanning C1 can significantly improve the apparent utilization rate of all essential amino acids and the total amount of essential amino acids (P<0.05), increasing the apparent utilization rates of lysine, methionine, tryptophan, arginine, histidine, leucine, isoleucine, phenylalanine, threonine, valine, and the total amount of essential amino acids by 3.63%, 3.57%, 6.28%, 6.45%, 3.03%, 2.96%, 2.14%, 4.72%, 2.03%, 1.76%, and 3.59%, respectively. 2) Non essential amino acids: Reducing the nutritional level of commercial broiler diets significantly improved the apparent utilization rate of cysteine (P<0.05), but="" had="" no="" significant="" effect="" on="" other="" indicators="" p="">0.05); The addition of Yanning C1 had no significant effect on tyrosine (P>0.05), but significantly increased the apparent utilization rates of other non essential amino acids and total amino acids (P<0.01), increasing the apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, and total non essential amino acids by 1.2%, 5.5%, 2.0%, 21.7%, 1.4%, 1.4%, 6.2%, and 4.0%, respectively.

Keywords: commodity broiler, Yanning C1, amino acid utilization rate

1. Materials and Methods

1.1 Test Materials

Yanning C1: a stress relieving formula product mainly composed of Boluohui and enzymatically hydrolyzed polysaccharides for anti-inflammatory and inflammatory repair purposes, provided by Shengdao Biotechnology (Shandong) Co., Ltd.

1.2 Experimental animals and design

1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); For treatments 3-4, add 500 mg/ton of Yanning C1 on top of treatments 1 and 2, respectively. The experimental chickens were fed pellet feed and allowed to freely feed and drink.

1.3 Experimental diet

Temporarily missing

1.4 Test Method

Feeding experiment: The experiment is divided into two stages: broiler chickens (0-21d) and broiler chickens (22-42d). Experimental chicken cage breeding, with 2 cages per replicate and 20 chickens per cage. Feed pellet feed.

1.5 Measurement indicators

Nutrient utilization efficiency:

Using the AIA indicator method, between 28-35 days, fecal samples were taken on a treatment basis to determine nutrient composition and calculate apparent utilization rate (amino acid utilization rate). (Note: During this period, select a day and before feeding in the morning. Within each replicate, collect fresh and uncontaminated feces of at least 100g per replicate, with 8 samples per treatment. At the same time, take 200g of the daily feed sample per treatment.)

1.6 Data statistics and analysis

The data was analyzed using SAS 9.2 software, and Duncan's multiple range test was used to compare the mean values, with a significance level of p<0.05.

2. Results and Analysis

2.1 Comparative analysis of apparent utilization rate of essential amino acids

Two levels of nutritional supplements (basal diet vs. reduced diet) were applied to the diet of commercial broiler chickens, corresponding to two levels of Yanning C1 supplementation (500mg/ton without VS supplementation). The effects on the apparent utilization efficiency of essential amino acids are shown in Tables 2 and 3. Analysis of variance showed that reducing the nutritional level of the diet for commercial broiler chickens had a trend of increasing the apparent utilization rate of methionine (P=0.059), but had no significant effect on other indicators (P>0.05); Adding Yanning C1 can significantly improve the apparent utilization rate of all essential amino acids and the total amount of essential amino acids (P<0.05); The nutritional level and Yanning C1 had an interactive effect on the apparent utilization rate of tryptophan (P<0.05), but="" had="" no="" interactive="" effect="" on="" other="" indicators="" p="">0.05). From a numerical comparison analysis, the apparent utilization rate of essential amino acids in the basic diet of commercial broiler chickens is 100%. After reducing the nutritional level of the diet, the apparent utilization rates of lysine, methionine, tryptophan, arginine, histidine, leucine, isoleucine, phenylalanine, threonine, valine, and the total amount of essential amino acids are 99.86%, 100.78%, 99.17%, 99.21%, 100.18%, 99.81%, 100.09%, 99.46%, 100.04%, 100.15%, and 99.89%, respectively; Without adding Yanning C1 as 100%, the apparent utilization rates of lysine, methionine, tryptophan, arginine, histidine, leucine, isoleucine, phenylalanine, threonine, valine, and essential amino acids were 103.63%, 103.57%, 106.28%, 106.45%, 103.03%, 102.96%, 102.14%, 104.72%, 102.03%, 101.76%, and 103.59%, respectively, after adding Yanning C1.

2.2 Comparative analysis of apparent utilization rates of non essential amino acids

Two levels of nutritional supplements (basal diet vs. reduced diet) were applied to the diet of commercial broiler chickens, corresponding to two levels of Yanning C1 supplementation (500mg/ton without VS supplementation). The effects on the apparent utilization of non essential amino acids are shown in Tables 4 and 5. Analysis of variance showed that reducing the nutritional level of the diet for commercial broiler chickens significantly improved the apparent utilization rate of cysteine (P<0.05), but="" had="" no="" significant="" effect="" on="" other="" indicators="" p="">0.05); The addition of Yanning C1 had no significant effect on tyrosine (P>0.05), but significantly increased the apparent utilization of other non essential amino acids and the total amount of amino acids (P<0.01); The nutritional level and Yanning C1 have an interactive effect on cysteine (P<0.05), and there is a trend of interactive effect on serine (P=0.090). From a numerical comparison analysis, the apparent utilization rate of non essential amino acids in the basic diet of commercial broiler chickens is 100%. After reducing the nutritional level of the diet, the apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, tyrosine, and total non essential amino acids are 99.94%, 100.38%, 99.92%, 100.36%, 99.94%, 100.46%, 101.45%, 100.55%, and 100.36%, respectively; Without adding Yanning C1 as 100%, the apparent utilization rates of aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine, tyrosine, and non essential amino acids were 101.2%, 105.5%, 102.0%, 121.7%, 101.4%, 101.4%, 106.2%, 99.9%, and 104.0%, respectively, after adding Yanning C1.

3. Conclusion

The analysis results showed that compared with the basic diet of commercial broiler chickens, there was no significant difference in the apparent utilization rate of essential and non essential amino acids after reducing the nutritional level of the diet; Adding Yanning C1 can improve the apparent utilization rate of all amino acids, which is beneficial for protein deposition in the body. Based on the comparative analysis of the four treatments, adding Yanning C1 to the commodity based diet resulted in the best apparent utilization rate of amino acids.

Table 1 Experimental Design

Serial Number	handle	Addition amount, mg/t	Repetitions	Chicken count/repetition	Total number of chickens
1	Positive control	0	8	40	320
2	Negative control	0	8	40	320
3	Positive control+Yanning C1	500	8	40	320
4	Negative control+Yanning C1	500	8	40	320

Table 2 Effect of Yanning C1 on the apparent utilization rate of essential amino acids in commercial broiler chickens

Dietary type	Yanning C1	Lysine	Methionine	Tryptophan	Arginine	Histidine	Leucine	Isoleucine	Phenylalanine	Threonine	Valine	TEAA
Apparent utilization rate,%
Commodity	-	86.43	84.55	78.95	77.59	84.65	85.78	88.06	85.59	90.27	88.51	85.04
Commodity	+	89.46	87.75	84.78	82.97	87.64	88.87	90.31	89.07	92.46	90.27	88.36
Decrement	-	86.20	85.39	79.14	77.34	85.21	86.17	88.51	84.58	90.66	88.85	85.20
Decrement	+	89.44	88.26	83.24	81.96	87.37	88.16	90.03	89.13	92.14	90.21	88.00
SEM		0.212	0.167	0.202	0.322	0.206	0.185	0.156	0.215	0.121	0.148	0.182
Pooled
Dietary type	Commodity	87.95	86.15	81.87	80.28	86.14	87.33	89.19	87.33	91.36	89.39	86.70
Dietary type	Decrement	87.82	86.82	81.19	79.65	86.29	87.16	89.27	86.86	91.40	89.53	86.60
Yanning C1	-	86.32	84.97	79.04	77.46	84.93	85.97	88.28	85.08	90.46	88.68	85.12
Yanning C1	+	89.45	88.00	84.01	82.46	87.51	88.52	90.17	89.10	92.30	90.24	88.18
p-value
Processing Room		＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001
Dietary type	Commodity vs decrement	0.772	0.059	0.109	0.337	0.714	0.666	0.792	0.285	0.881	0.648	0.789
Yanning C1	Increase vs Do not add	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001
Dietary type vs Yanning C1		0.798	0.629	0.045	0.563	0.325	0.152	0.258	0.225	0.156	0.514	0.477

Table 3 Relative comparison of the effects of Yanning C1 on the apparent utilization rate of essential amino acids in commercial broiler chickens,%

Dietary type	Yanning C1	Lysine	Methionine	Tryptophan	Arginine	Hhistidine	Leucine	Isoleucine	Phenylalanine	Threonine	Valine	TEAA
Commodity	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Commodity	+	103.50	103.78	107.39	106.93	103.53	103.61	102.56	104.06	102.44	101.99	103.91
Decrement	-	99.73	100.98	100.24	99.67	100.67	100.45	100.51	98.82	100.44	100.38	100.19
Decrement	+	103.48	104.38	105.43	105.63	103.22	102.78	102.24	104.14	102.08	101.92	103.48
Pooled
Dietary type	Commodity	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Dietary type	Decrement	99.86	100.78	99.17	99.21	100.18	99.81	100.09	99.46	100.04	100.15	99.89
Yanning C1	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Yanning C1	+	103.63	103.57	106.28	106.45	103.03	102.96	102.14	104.72	102.03	101.76	103.59

Table 4 Effect of Yanning C1 on the apparent utilization rate of non essential amino acids in commercial broiler chickens

Dietary type	Yanning C1	Aspartate	Serine	Glutamate	Proline	Glycine	Alanine	Cystine	Tyrosine	TNEAA
Apparent utilization rate,%
Commodity	-	92.81	78.15	90.96	55.37	92.69	89.38	78.68	92.87	83.86
Commodity	+	94.14	83.34	92.60	68.56	93.99	90.77	85.07	93.13	87.70
Decrement	-	92.94	79.35	90.71	56.61	92.68	89.90	81.31	93.73	84.66
Decrement	+	93.91	82.75	92.70	67.77	93.88	91.08	84.81	93.29	87.52
SEM		0.086	0.252	0.097	0.546	0.151	0.134	0.199	0.164	0.176
Pooled
Dietary type	Commodity	93.48	80.74	91.78	61.97	93.34	90.07	81.87	93.00	85.78
Dietary type	Decrement	93.42	81.05	91.71	62.19	93.28	90.49	83.06	93.51	86.09
Yanning C1	-	92.88	78.75	90.83	55.99	92.69	89.64	80.00	93.30	84.26
Yanning C1	+	94.02	83.05	92.65	68.16	93.94	90.93	84.94	93.21	87.61
p-value
Processing Room		＜0.001	＜0.001	＜0.001	＜0.001	0.005	＜0.001	＜0.001	0.3334	＜0.001
Dietary type	Commodity vs Decrement	0.749	0.546	0.717	0.840	0.847	0.137	0.007	0.137	0.392
Yanning C1	Increase vs Do not add	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	＜0.001	0.795	＜0.001
Dietary type vs Yanning C1		0.318	0.090	0.379	0.362	0.859	0.690	0.002	0.298	0.184

Table 5: Relative comparison of the effects of Yanning C1 on the apparent utilization rate of non essential amino acids in commercial broiler chickens,%

Dietary type	Yanning C1	Aspartate	Serine	Glutamate	Proline	Glycine	Alanine	Cystine	Tyrosine	TNEAA
Commodity	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Commodity	+	101.43	106.65	101.80	123.82	101.41	101.56	108.11	100.28	104.58
Decrement	-	100.13	101.54	99.73	102.24	99.99	100.59	103.35	100.93	100.94
Decrement	+	101.18	105.89	101.92	122.38	101.28	101.90	107.79	100.46	104.36
Pooled
Dietary type	Commodity	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Dietary type	Decrement	99.94	100.38	99.92	100.36	99.94	100.46	101.45	100.55	100.36
Yanning C1	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Yanning C1	+	101.24	105.46	102.00	121.74	101.35	101.43	106.18	99.91	103.98