Research on the Application of Stress Ning Commercial Broiler Production Performance - Scientific Payoffs

Abstract: The purpose of this experiment is to study the application efficacy of stress Ning on the production performance of commercial broiler chickens. 1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); Process 3-4, add stress Ning 500 mg/ton on the basis of Process 1 and 2 respectively. The experiment is divided into two stages (0-21d, 22-42d). Experimental chicken cage feeding, feeding pellet feed, with consistent dietary composition and nutritional levels between treatments.

The research results showed that compared with no addition, adding stress Ning to the diet of commercial broiler chickens significantly increased daily weight gain by 22-42 days and 42 day slaughter weight, reduced feed to weight ratio (p<0.05), and showed a trend of increasing 21 day weight (P=0.086) and decreasing 1-21 day feed to weight ratio (P=0.055); However, compared with the basic diet, the reduction in nutritional levels increased the feed to weight ratio by 1-21d and 22-42d (p<0.05), with a trend of decreasing daily weight gain by 1-21d (P=0.092) and 22-42d (P=0.077). From the entire experimental period (1-42 days), the addition of stress Ning significantly increased daily weight gain and European index, while reducing feed to gain ratio (p<0.05); The reduction in nutritional levels resulted in a decrease in daily weight gain and European index (p<0.05), with a trend towards increasing feed to weight ratio (P=0.057). Adding stress Ning to the commodity diet increased daily weight gain, daily feed intake, 42 day slaughter weight, and European index by 1.47%, 0.08%, 2.04%, and 1.13%, respectively, from day 1 to day 42. The feed to weight ratio decreased by 1.39%. Based on the slaughter weight and feed consumption cost of broiler chickens, an economic benefit evaluation was conducted. Compared with the control group, the net benefits of each chicken in the positive control+stress Ning group and the negative control+stress Ning group increased by 0.31 yuan and 0.15 yuan, respectively; The reduction in nutritional levels has lowered economic benefits, resulting in a loss of 0.15 yuan per chicken.

Conclusion: Adding stress Ning to the diet of commercial broiler chickens has the effect of improving production performance and increasing breeding income.

Keywords: commodity broiler, stress Ning, production performance, economic benefits

1. Materials and Methods

1.1 Test Materials

Stress Ning: An anti stress formula product mainly composed of star anise ether for antioxidant and immune enhancement effects, provided by SD BIOTECH GROUP.

1.2 Experimental animals and design

1280 healthy 1-day-old AA broiler chickens with similar body weight were selected and randomly divided into 4 treatments, with 8 replicates per treatment and 40 chickens per replicate (see Table 1). Process 1: Positive control group, commercial feed; Treatment 2: Negative control group. On the basis of the control group, the nutritional level of the formula was reduced according to the reduction plan (metabolic energy decreased by 30 kcal/kg, crude protein decreased by 0.5%); Process 3-4, add stress Ning 500 mg/ton on the basis of Process 1 and 2 respectively. The experimental chickens were fed pellet feed and allowed to freely feed and drink.

1.3 Experimental diet

Temporarily missing

1.4 Test Method

Feeding experiment: The experiment is divided into two stages: broiler chickens (0-21d) and broiler chickens (22-42d). Experimental chicken

Caged, each replicate consists of 2 cages, with 20 animals per cage. Feed pellet feed.

1.5 Measurement indicators

Production performance: Record feed intake daily and weigh at 21 and 42 days. Calculate the average daily feed intake, average daily weight gain, and feed to gain ratio. Record mortality rate, diarrhea rate, stool characteristics, mental state, etc. Calculate the European index.

1.6 Data statistics and analysis

The data was analyzed using SAS 9.2 software, and Duncan's multiple range test was used to compare the mean values, with a significance level of p<0.05.

2. Results and Analysis

2.1 Comparative analysis of production performance

Two levels of nutritional supplements (basal diet vs. reduced diet) were added to the diet of commercial broiler chickens, corresponding to two levels of stress Ning supplementation (500mg/ton without VS supplementation). The effects on the production performance of broiler chickens are shown in Tables 2 and 3. Analysis of variance showed that, compared with no addition, adding stress Ning to the diet of commercial broiler chickens significantly increased daily weight gain from 22 to 42 days and slaughter weight from 42 days, while reducing feed to weight ratio (p<0.05). There was a trend of increasing body weight by 21 days (P=0.086) and decreasing feed to weight ratio by 1-2 days (P=0.055); Compared with the basic diet, reducing nutrient levels increased the feed to weight ratio by 1-21d and 22-42d (p<0.05), with a trend of decreasing daily weight gain by 1-21d (P=0.092) and 22-42d (P=0.077). From the whole period (1-42 days), the addition of stress Ning significantly increased daily weight gain and European index, while reducing feed to gain ratio (p<0.05); The reduction in nutritional levels resulted in a decrease in daily weight gain and European index (p<0.05), with="" a="" trend="" towards="" increasing="" feed="" to="" weight="" ratio="" p="" there="" is="" of="" interaction="" between="" nutritional="" level="" and="" stress="" tolerance="" on="" the="" 22-42d="" broiler="" chickens="" but="" no="" significant="" effect="" other="" indicators="">0.05).

From a numerical comparison analysis, the production performance indicators of the positive control group (basic feed without adding stress Ning) were 100%. Based on the comprehensive comparison of the whole period (1-42 days), after adding stress Ning to the basic feed, the daily weight gain, daily feed intake, feed to weight ratio, 42 day slaughter weight, and European index were 101.47%, 100.08%, 98.61%, 102.04%, and 101.13%, respectively; The daily weight gain, feed intake, feed to weight ratio, 42 day slaughter weight, and European index of the negative control group (reduced feed without adding stress Ning) were 97.54%, 99.51%, 102.10%, 97.75%, and 96.18%, respectively. After adding stress Ning to the reduced feed, the daily weight gain, feed to weight ratio, 42 day slaughter weight, and European index were 99.46%, 99.78%, 100.34%, 99.84%, and 98.72%, respectively.

It can be seen that adding stress Ning to the diet of commercial broiler chickens can significantly improve production performance, reduce feed to weight ratio, improve feed efficiency, and achieve better economic benefits.

2.2 Economic Benefit Analysis

According to Table 4, compared with the positive control group, the feed consumption of the negative control group (nutrient water reduction), positive control+stress Ning group, and negative control+stress Ning group increased by -0.01, 0.01, and 0 kg/animal, respectively, and the slaughter weight of the broiler chickens increased by -0.05, 0.06, and 0 kg/animal, respectively. Based on the weight of broiler chickens slaughtered and the cost of feed consumption, an economic benefit evaluation was conducted. Compared with the control group, the net benefits of each chicken in the positive control+stress Ning group and the negative control+stress Ning group increased by 0.31 yuan and 0.15 yuan, respectively. Calculated as per 10 million chickens, the net benefits can be increased by 3.1349 million yuan and 1.4578 million yuan, respectively; The reduction in nutritional levels has lowered economic benefits, resulting in a loss of 0.15 yuan per chicken.

3. Conclusion

Compared with the control group, the addition of stress Ning to the basal diet significantly improved production performance, resulting in a 2.04% increase in 42 day slaughter weight and a 1.39% decrease in feed to weight ratio; At the same time, it obtained the best European index, improved breeding income, and increased net profit per chicken by 0.31 yuan. The reduction of nutritional levels in commercial broiler feed may have adverse effects on the production performance of broiler chickens, and the detailed mechanism needs further research and exploration.

Table 1 Experimental Design

Serial Number	Handle	Addition amount, mg/t	Repetitions	Chicken count/Repetition	Total number of chickens
1	Positive control	0	8	40	320
2	Negative control	0	8	40	320
5	Positive control+Stress Ning	500	8	40	320
6	Negative control+Stress Ning	500	8	40	320

Table 2 The Effect of Stress Ning on the Production Performance of Commercial Broiler Chickens

Type of diet	Stress Ning	1-21d				22-42d				1-42d
Type of diet	Stress Ning	ADG,g/d/only	ADFI,g/d/only	F/G	21d BW,g/only	ADG,g/d/only	ADFI,g/d/only	F/G	42d BW,g/only	ADG,g/d/only	ADFI,g/d/only	F/G	MSCI Europe,EBI
Commodity	-	43.26	48.23	1.11	949.73	85.38	132.90	1.56	2565.00	63.27	88.45	1.40	449.59
Commodity	+	43.58	47.97	1.10	959.69	87.36	133.95	1.53	2617.38	64.20	88.52	1.38	454.67
Decrement	-	42.77	48.00	1.12	941.79	82.34	133.41	1.62	2507.21	61.71	88.02	1.43	432.40
Decrement	+	42.88	48.21	1.12	952.40	85.07	132.18	1.55	2560.80	62.93	88.25	1.40	443.83
SEM		0.171	0.151	0.003	3.832	0.730	0.954	0.007	17.563	0.404	0.481	0.005	4.302
Pooled
Type of diet	Commodity	43.42	48.10	1.11	954.71	86.37	133.43	1.55	2591.19	63.73	88.49	1.39	452.13
Type of diet	Decrement	42.82	48.11	1.12	947.10	83.70	132.80	1.59	2534.01	62.32	88.14	1.42	438.11
Stress Ning	-	43.02	48.12	1.12	945.76	83.86	133.16	1.59	2536.10	62.49	88.23	1.41	440.99
Stress Ning	+	43.23	48.09	1.11	956.05	86.22	133.07	1.54	2589.09	63.56	88.39	1.39	449.25
P - value
Processing Room		0.190	0.376	0.207	0.428	0.288	0.925	0.030	0.126	0.433	0.983	0.068	0.312
Stress Ning	Increase vs Do not add	0.547	0.927	0.055	0.086	0.042	0.963	0.002	0.031	0.032	0.874	0.028	0.013
Type of diet	Commodity vs Decrement	0.092	0.980	0.009	0.328	0.077	0.744	0.003	0.113	0.027	0.718	0.057	0.004
Type of diet VS Stress Ning		0.757	0.446	0.159	0.966	0.800	0.555	0.089	0.986	0.863	0.933	0.789	0.715

Table 3 Comparison of Relative Values of the Effects of Stress Ning on the Production Performance of Commercial Broiler Chickens,%

Type of diet	Stress Ning	1-21d				22-42d				1-42d
Type of diet	Stress Ning	ADG g/d/only	ADFI g/d/only	F/G	21d BW g/only	ADG g/d/only	ADFI g/d/only	F/G	42d BW g/only	ADG g/d/only	ADFI g/d/only	F/G	MSCI Europe EBI
Commodity	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Commodity	+	100.73	99.46	98.75	101.05	102.32	100.79	98.43	102.04	101.47	100.08	98.61	101.13
Decrement	-	98.87	99.53	100.68	99.16	96.44	100.38	104.16	97.75	97.54	99.51	102.10	96.18
Decrement	+	99.10	99.96	100.87	100.28	99.64	99.46	99.82	99.84	99.46	99.78	100.34	98.72
Pooled
Type of diet	Commodity	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Type of diet	Decrement	98.63	100.02	101.41	99.20	96.91	99.53	102.79	97.79	97.78	99.60	101.93	96.90
Stress Ning	-	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100.00
Stress Ning	+	100.48	99.94	99.47	101.09	102.81	99.93	97.11	102.09	101.71	100.17	98.44	101.87

Table 4 Economic Benefit Analysis (Referring to Average Prices in Previous Years)

Items	Positive control	Negative control	Positive control + Stress Ning	Negative control + Stress Ning
Chicken fry unit price,RMB/piece	4	4	4	4
Feed intake, g/piece/day	88.45	88.02	88.52	88.25
Feed consumption, kg/piece	3.71	3.70	3.72	3.71
Feed unit price,RMB/kg	3.5	3.44	3.52	3.46
Feed cost,RMB/piece	13.00	12.72	13.09	12.82
Average daily weight gain,g/d	63.27	61.71	64.20	62.93
Weight of slaughtered chickens,kg/each	2.56	2.51	2.62	2.56
Raw chicken price,RMB/kg	7.6	7.6	7.6	7.6
Chicken income,RMB	19.49	19.05	19.89	19.46
Generate benefits,RMB/piece	2.49	2.34	2.81	2.64
Stress Ning produces benefits,RMB/piece	—	-0.15	0.31	0.15
Stress Ning produces benefits,RMB/10000 pieces	—	-1500	3134.91	1457.83
Stress Ning produces benefits,10000 RMB/million pieces	—	-150	313.49	145.78