Summary:

The purpose of this experiment is to investigate the degradation efficiency of fungal toxin content in sugar residue by mycolytic enzyme. 100g of sugar residue was crushed in a grinder and 30mg of mycolytic enzyme was added. Add an aqueous solution to fully dissolve both, stir evenly, and take samples for testing at 0 hours, 30 minutes, 1 hour, and 2 hours in a 37 ℃ water bath. The test results indicate that:

Due to the low levels of three types of mycotoxins in the sugar residue sample, the content of aflatoxin B1 after treatment with mycolytic enzymes was also lower than the minimum detection limit.

1、Materials and Methods

1.1 Test Materials

Fungal hydrolysis enzyme sample: SD BIOTECH GROUP

Corn samples

1.2 Test Method

Sample pretreatment: Take 100g of sugar residue and crush it in a grinder, then add 30mg of mycolytic enzyme. Add aqueous solution to fully dissolve both and stir evenly. 37 ℃ water bath was used, and samples were taken at 0h, 30min, 1h, and 2h for testing. 5g of the test sample was weighed and placed in a 50mL centrifuge tube. 25mL of 60% ethanol extract was added and vortexed for 3 minutes. The tube was then centrifuged at 5000rpm for 5 minutes. 100 μ L of the supernatant was taken into a new centrifuge tube, and 500 μ L of sample diluent was added and mixed. The fluorescence quantitative detector was turned on, preheated for 5 minutes, and the incubator was opened. The temperature was raised to 37 ℃, and 100 μ L of the mixed sample was taken and added to the sample well of the detection card. The tube was incubated for 8 minutes, and the detection card was inserted into the detection slot for testing.

2、Experimental results

Table 1 Fungal toxin content in sugar residue

3、Experimental conclusion

Due to the low levels of three types of mycotoxins in the sugar residue sample, the content of aflatoxin B1 after treatment with mycolytic enzymes was also lower than the minimum detection limit.