Summary:

The purpose of this experiment is to explore the degradation efficiency of zearalenone in corn by different amounts of mycolytic enzymes using a rapid detection method. 200g of corn was taken and crushed in a grinder, and then divided into two parts, each weighing 100g; One group added fungal hydrolysis enzyme sample at a rate of 0.25g/ton, and the other group added fungal hydrolysis enzyme sample at a rate of 0.5g/ton. 37 ℃ water bath, and samples were taken for testing at 0h, 30min, 1h, and 2h. The test results indicate that:

Because the content of zearalenone in the corn sample exceeded the detection range, with zearalenone content>1000ppb, the degradation rate was calculated based on 1000ppb as a control. A group was added with 0.25g/ton of cellulase, and the degradation rate of zearalenone in corn was 62.17% after 30 minutes, 70.05% after 1 hour, and 88.09% after 2 hours; The degradation rate of zearalenone in corn was 67.07% after 30 minutes, 73.47% after 1 hour, and 93.23% after 2 hours, with the addition of fungal enzymes at a rate of 0.5g/ton in two groups.

1、Materials and Methods

1.1 Test Materials

Fungal hydrolysis enzyme sample: provided by SD BIOTECH GROUP

Corn sample: provided by a company in the Henan market

1.2 Test Method

Sample pretreatment: Take 200g of corn and grind it into two pieces of 100g each. One group will be treated with 0.25g/ton of fungal hydrolysis enzyme sample, and the other group will be treated with 0.5g/ton of fungal hydrolysis enzyme sample. Add 75mL of aqueous solution to dissolve thoroughly and stir evenly. 37 ℃ water bath, and samples were taken for testing at 0h, 30min, 1h, and 2h. Weigh 5g of the test sample into a 50mL centrifuge tube. Add 25mL of 60% ethanol extract, vortex and mix for 3 minutes, centrifuge at 5000rpm for 5 minutes at room temperature. Take 100 μ L of supernatant into a new centrifuge tube, then add 500 μ L of sample diluent and mix well. Turn on the fluorescence quantitative detector and preheat for 5 minutes. Open the incubator and raise the temperature to 37 ℃. Take 100 μ L of mixed sample and add it to the detection card well. React in the incubator for 8 minutes. Insert the detection card into the detection slot for testing.

2、Experimental results:

Table 1 Content of zearalenone in corn

Figure 1-Degradation rate of zearalenone content in maize by a group of fungal enzymes

Figure 2- 2 Degradation rate of zearalenone content in maize by two groups of mycolytic enzymes

3、Experimental conclusion

Because the content of zearalenone in the corn sample exceeded the detection range, with zearalenone content>1000ppb, the degradation rate was calculated based on 1000ppb as a control. A group was added with 0.25g/ton of cellulase, and the degradation rate of zearalenone in corn was 62.17% after 30 minutes, 70.05% after 1 hour, and 88.09% after 2 hours; The degradation rate of zearalenone in corn was 67.07% after 30 minutes, 73.47% after 1 hour, and 93.23% after 2 hours, with the addition of fungal enzymes at a rate of 0.5g/ton in two groups.