ØMix the product ingredients of zearalenone degrading enzyme and vomitoxin degrading enzyme to prepare a mixed enzyme solution with concentrations of 30U/g (ZEN degrading enzyme) and 50U/g (DON degrading enzyme).
ØWeigh 20g of corn kernels from the market and place them in 250mL triangular flasks. Add 20mL of enzyme solution and mix well. React at room temperature (25-28 ℃) for 4h, 8h, and 16h, respectively. After the reaction is complete, take a sample to test the toxin content.
ØWeigh a certain amount of the sample, grind it and sieve it through a 30 mesh sieve. Weigh 20g of the crushed sample into a 250mL triangular flask, add 20mL of enzyme solution, and react at room temperature (25-28 ℃) for 4h, 8h, and 16h, respectively. After the reaction is complete, take a sample to test the toxin content.
Result: Under experimental conditions, the enzyme solution had a very limited effect on the degradation of toxins in whole corn. The possible reason for this is that the whole corn cannot fully contact with the degrading enzyme, resulting in the inability of toxins to be degraded. The degradation effect of MuNing Dulijing on crushed samples is significant.
Serial number | Sample Name | Reaction time h | Enzyme solution concentration U/g | Reaction volume mL | DON degradation amount ug | ZEN degradation amount ug | DON degradation rate | ZEN degradation rate |
1 | Whole grain corn | 4 | 30(ZEN) 、50(DON) | 20 | 6.2 | 1.44 | 8% | 11% |
3 | 8 | 30(ZEN) 、50(DON) | 20 | 11.1 | 1.51 | 15% | 11% | |
5 | 16 | 30(ZEN) 、50(DON) | 20 | 11.9 | 1.53 | 16% | 12% | |
13 | Crushed corn | 4 | 30(ZEN) 、50(DON) | 20 | 66.7 | 10.44 | 88% | 79% |
15 | 8 | 30(ZEN) 、50(DON) | 20 | 67.2 | 10.48 | 89% | 80% | |
17 | 16 | 30(ZEN) 、50(DON) | 20 | 66.8 | 10.4 | 88% | 79% |
