Weigh 10g of corn substrate from a certain company in the market and place it in three volumetric flasks. After testing, the content of aflatoxin B1, vomitoxin, and zearalenone in the samples was 30ug/kg, 3mg/kg, and 0.5mg/kg, respectively. Add 0.1mg of mycophenolate mofetil and 20g of artificial simulated gastric acid, shake and mix evenly at 39 ℃, and take samples to detect the toxin content at 1h, 2h, and 4h of reaction.

Mix the product ingredients of zearalenone degrading enzyme and vomitoxin degrading enzyme to prepare a mixed enzyme solution with concentrations of 30U/g (ZEN degrading enzyme) and 50U/g (DON degrading enzyme).

The purpose of this experiment is to explore the degradation efficiency of zearalenone in corn by different amounts of mycolytic enzymes using a rapid detection method. 200g of corn was taken and crushed in a grinder, and then divided into two parts, each weighing 100g; One group added fungal hydrolysis enzyme sample at a rate of 0.25g/ton, and the other group added fungal hydrolysis enzyme sample at a rate of 0.5g/ton. 37 ℃ water bath, and samples were taken for testing at 0h, 30min, 1h, and 2h.

The purpose of this experiment is to investigate the degradation efficiency of fungal toxin content in sugar residue by mycolytic enzyme. 100g of sugar residue was crushed in a grinder and 30mg of mycolytic enzyme was added. Add an aqueous solution to fully dissolve both, stir evenly, and take samples for testing at 0 hours, 30 minutes, 1 hour, and 2 hours in a 37 ℃ water bath.

The purpose of this experiment is to investigate the degradation efficiency of vomitoxin content in corn by different amounts of fungal enzymes. 200g of corn was taken and crushed in a grinder, and then divided into two parts, each weighing 100g; Add 50mg of mycolytic enzyme to one of the two samples, and record as one group; Add another 25mg of mycolytic enzyme and record as 2 groups. 37 ℃ water bath, and samples were taken for testing at 0h, 2h, and 4h.

The 4th Feed Industry New Product and Technology Seminar of 2023 was successfully held at Shunhe International Hotel in Jinan from December 11th to December 12th, 2023. After independent application by the enterprise, media visits, expert evaluations, and other processes, our company's application for the combination of Aspergillus flavus toxin degrading enzyme and mannan oligosaccharides as a mixed feed additive has been awarded the "2023 Green and Efficient Feed Demonstration Product".

(1) 202210377945. X, a plant extract fungal biological preparation for anti-inflammatory and growth promoting use in livestock and poultry, and its preparation method;

SD BIOTECH GROUP has collaborated with Shandong Livestock Product Safety and Quality Center, Shandong Hemei Agriculture and Animal Husbandry Technology Co., Ltd., and Shandong Boon Feed Group Co., Ltd. to develop and industrialize five technological achievements, including "Research and Industrialization Application of Antibiotic Free and Efficient Feed for Meat and Poultry", "Research and Demonstration of Key Technologies for High Quality Functional Egg Production", "Nutritional Optimization and Functional Premix Development and Application for Egg Chickens", "Industrialization Application of Antibiotic Free Feed and Alternative Antibiotic Breeding for Pigs", and "Development of Antibiotic Free Feed for Pigs and Its Application in High Quality Pork Production". Four achievements have been rated as international advanced level, and one achievement has been rated as domestic leading level.

The overall technology of the research and application of key technologies for high and stable production of high-quality milk, in which SD BIOTECH GROUP is deeply involved, has reached the international advanced level.

On July 27, 2022, the Shandong Society of Animal Husbandry and Veterinary Medicine organized experts to evaluate the achievements of SD BIOTECH GROUP in the research and demonstration of key technologies for the anti stress effect and industrialization of Boluohui extract. The expert group listened to the project team's report, reviewed relevant materials, and after questioning and answering questions, the expert group unanimously agreed that the overall technology of the project has reached the international advanced level.